Quantifying cell-ECM adhesion by single cell force spectroscopy - Quantifying adhesive interactions between cells and extracellular matrix by atomic force microscopy based single cell force spectroscopy
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Lieferung aus: Deutschland, Versandkostenfrei.
Von Händler/Antiquariat, European-Media-Service Mannheim [1048135], Mannheim, Germany.
Publisher/Verlag: Südwestdeutscher Verlag für Hochschulschriften | Quantifying adhesive interactions between cells and extracellular matrix by atomic force microscopy based single cell force spectroscopy | Integrin-mediated adhesive interactions of cells with the extracellular matrix (ECM) regulate important cellular functions and play a critical role in disease. Therefore, studying the mechanisms underlying integrin-binding and regulation is of great interest. In the presented work three respective biological questions have been addressed using Atomic Force Microscopy based Single Cell Force Spectroscopy (AFM-SCFS). The work is split into six chapters. The first chapter provides an introduction into cell-ECM interactions. Thereafter different adhesion assays are compared and contrasted, and the theoretical Bell-Evans model is discussed. In chapter four, alpha2beta1-integrin mediated cell adhesion to collagen I is quantified. Thereafter the effects of the exposure of cryptic integrin binding sites within collagen I on pre-osteoblasts are analysed. At last, the role of BCR/ABL in modulating myeloid progenitor cell adhesion to stromal cells and ECM-coated substrates is studied. Taken together, the work demonstrates that AFM-SCFS is a versatile tool that permits monitoring of cell adhesion from single-molecule recognition events to the formation of more complex adhesion sites. | Format: Paperback | Language/Sprache: english | 184 pp.

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Von Händler/Antiquariat, Amazon.com.
Integrin-mediated adhesive interactions of cells with the extracellular matrix (ECM) regulate important cellular functions and play a critical role in disease. Therefore, studying the mechanisms underlying integrin-binding and regulation is of great interest. In the presented work three respective biological questions have been addressed using Atomic Force Microscopy based Single Cell Force Spectroscopy (AFM-SCFS). The work is split into six chapters. The first chapter provides an introduction into cell-ECM interactions. Thereafter different adhesion assays are compared and contrasted, and the theoretical Bell-Evans model is discussed. In chapter four, alpha2beta1-integrin mediated cell adhesion to collagen I is quantified. Thereafter the effects of the exposure of cryptic integrin binding sites within collagen I on pre-osteoblasts are analysed. At last, the role of BCR/ABL in modulating myeloid progenitor cell adhesion to stromal cells and ECM-coated substrates is studied. Taken together, the work demonstrates that AFM-SCFS is a versatile tool that permits monitoring of cell adhesion from single-molecule recognition events to the formation of more complex adhesion sites. Paperback, Label: Suedwestdeutscher Verlag fuer Hochschulschriften, Suedwestdeutscher Verlag fuer Hochschulschriften, Produktgruppe: Book, Publiziert: 2011-03-06, Studio: Suedwestdeutscher Verlag fuer Hochschulschriften.

Lieferung aus: Vereinigte Staaten von Amerika, Versandkosten nach: DEU.
Von Händler/Antiquariat, Ergodebooks.
Suedwestdeutscher Verlag fuer Hochschulschriften, 2011-03-06. Paperback. Good.

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Von Händler/Antiquariat, FastMedia "Versenden von USA"
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Von Händler/Antiquariat, MARZIES.de Buch- und Medienhandel, 14621 Sch.
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