Advanced Methods in Protein Sequence Determination (Molecular Biology, Biochemistry and Biophysics Molekularbiologie, Biochemie und Biophysik)
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Von Händler/Antiquariat, Phatpocket Limited.
Springer-Verlag. Used - Good. Ships from UK in 48 hours or less (usually same day). Your purchase helps support the African Children's Educational Trust (A-CET). Ex-library, so some stamps and wear, but in good overall condition. 100% money back guarantee. We are a world class secondhand bookstore based in Hertfordshire, United Kingdom and specialize in high quality textbooks across an enormous variety of subjects. We aim to provide a vast range of textbooks, rare and collectible books at a great price. Through our work with A-CET we have helped give hundreds of young people in Africa the vital chance to get an education. We provide a 100% money back guarantee and are dedicated to providing our customers with the highest standards of service in the bookselling industry.

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buecher.de GmbH & Co. KG, [1].
Confusion now hath made his masterpiece Macbeth II iii 72 Whence and what are those execrable shape? Paradise Lost Ib 1 681 Confusion worse confounded Paradise Lost Ib 1 995 When the manuscript for the first part of this book was proposed, it was anticipated that the discussion of the entire field of protein se quencing could be covered in a single volume - from purification and characterization of the protein through fragmentation by chemical or enzymic means and, finally, to reassembly of the identified individual peptides into the reconstructed total sequence. It soon became evident that this would not be possible. While the intent was to restrict the expose of procedures only to that information concerned with "hands on" wet chemistry, it became apparent that a thorough presentation would require, in addition, a discussion of certain instrumental and more theoretical approaches not included in the first volume. Furthermore, the entire understanding of the field of protein sequencing has advanced appreciably since the inception of this book. The purpose of the first volume was to provide practical information in sufficient detail to permit the researcher to undertake the actual sequencing procedures in his own laboratory.Softcover reprint of the original 1st ed. 1977. 2012. 22 SW-Abb.,. 244 mmVersandfertig in 3-5 Tagen, Softcover.

Lieferung aus: Österreich, Lagernd.
Confusion now hath made his masterpiece Macbeth II iii 72 Whence and what are those execrable shape? Paradise Lost Ib 1 681 Confusion worse confounded Paradise Lost Ib 1 995 When the manuscript for the first part of this book was proposed, it was anticipated that the discussion of the entire field of protein se­ quencing could be covered in a single volume - from purification and characterization of the protein through fragmentation by chemical or enzymic means and, finally, to reassembly of the identified individual peptides into the reconstructed total sequence. It soon became evident that this would not be possible. While the intent was to restrict the expose of procedures only to that information concerned with "hands on" wet chemistry, it became apparent that a thorough presentation would require, in addition, a discussion of certain instrumental and more theoretical approaches not included in the first volume. Furthermore, the entire understanding of the field of protein sequencing has advanced appreciably since the inception of this book. The purpose of the first volume was to provide practical information in sufficient detail to permit the researcher to undertake the actual sequencing procedures in his own laboratory. Soft cover.

Lieferung aus: Österreich, Lagernd.
Confusion now hath made his masterpiece Macbeth II iii 72 Whence and what are those execrable shape? Paradise Lost Ib 1 681 Confusion worse confounded Paradise Lost Ib 1 995 When the manuscript for the first part of this book was proposed, it was anticipated that the discussion of the entire field of protein se­ quencing could be covered in a single volume - from purification and characterization of the protein through fragmentation by chemical or enzymic means and, finally, to reassembly of the identified individual peptides into the reconstructed total sequence. It soon became evident that this would not be possible. While the intent was to restrict the expose of procedures only to that information concerned with "hands on" wet chemistry, it became apparent that a thorough presentation would require, in addition, a discussion of certain instrumental and more theoretical approaches not included in the first volume. Furthermore, the entire understanding of the field of protein sequencing has advanced appreciably since the inception of this book. The purpose of the first volume was to provide practical information in sufficient detail to permit the researcher to undertake the actual sequencing procedures in his own laboratory. eBook.

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Von Händler/Antiquariat, Better World Books: North.
Confusion now hath made his masterpiece Macbeth II iii 72 Whence and what are those execrable shape? Paradise Lost Ib 1 681 Confusion worse confounded Paradise Lost Ib 1 995 When the manuscript for the first part of this book was proposed, it was anticipated that the discussion of the entire field of protein se­ quencing could be covered in a single volume - from purification and characterization of the protein through fragmentation by chemical or enzymic means and, finally, to reassembly of the identified individual peptides into the reconstructed total sequence. It soon became evident that this would not be possible. While the intent was to restrict the expose of procedures only to that information concerned with "hands on" wet chemistry, it became apparent that a thorough presentation would require, in addition, a discussion of certain instrumental and more theoretical approaches not included in the first volume. Furthermore, the entire understanding of the field of protein sequencing has advanced appreciably since the inception of this book. The purpose of the first volume was to provide practical information in sufficient detail to permit the researcher to undertake the actual sequencing procedures in his own laboratory. Hardcover, Label: Springer-Verlag, Springer-Verlag, Produktgruppe: Book, Publiziert: 1977-06, Studio: Springer-Verlag, Verkaufsrang: 15622283.

Von Händler/Antiquariat, AHA-BUCH GmbH [51283250], Einbeck, NDS, Germany.
This item is printed on demand - Print on Demand Titel. Neuware - 1 Step-wise Degradation of Peptides Attached to Solid Supports.- I. Introduction.- II. Choice of Solid Support.- A. Polystyrene Beads.- B. Glass Beads.- III. Attachment of Peptides to Supports.- A. Carboxyl Activation Methods.- B. Amino Group Coupling.- IV. Step-wise Degradation Procedures.- A. Isothiocyanate Methods.- B. Thioacetylation Method.- V. Automation of Solid Phase Degradation.- A. Semi-Automated Operation.- B. An Inexpensive Automated Apparatus.- VI. Concluding Remarks.- 2 Coupling Methods and Strategies in Solid-Phase Sequencing.- I. Introduction.- II. Coupling Procedures.- A. Attachment by Activation of Carboxyl Groups.- B. Attachment of Amino Side Chains Using p-Phenylene Diisothiocyanate.- C. Combined Methods of Attachment.- D. Sequencing Resins.- E. Summary of Attachment Procedures.- III. Sequencing Strategies.- 3 Sequencing Peptides and Proteins Lacking Free -Amino Groups.- I. Introduction.- II. Isolation and Detection of Peptides Lacking Free -Amino Groups.- III. PCA-Terminating Peptides and Proteins.- A. Chemical Approaches.- B. Enzymatic Removal of PCA Residues.- IV. Acylated Peptides and Proteins.- A. Chemical Identification Procedures.- B. Enzymatic Removal of Acetylated Amino-Terminal Residues.- V. Concluding Remarks.- 4 Use of Antibody in the Study of Protein Structure.- I. Introduction.- II. Measurement of the Ag-Ab Reaction.- III. The Effect of Conformational Changes on the Antigenicity of Proteins.- IV. Detection of Sequence Changes in Proteins with Antibodies.- V. Use of Antibody in the Study of Protein Evolution.- 5 Polarization of Light and Protein Structure.- I. Introduction.- II. Plane Polarized Light.- III. Circularly Polarized Light.- IV. Circular Dichroism.- V. Drude Equation.- VI. Optical Properties of Amino Acids.- VII. Optical Properties of Peptides.- VIII. Conclusion.- 6 The X-ray Crystallography Technique in Protein Sequencing.- I. Introduction.- II. Outline of the Protein Crystallographic Method.- III. Some Important Concepts in X-ray Diffraction Analysis of Protein Crystals.- IV. Building up the Electron Density Map.- V. Interpretation of the Electron Density Map.- A. Molecular Boundary.- B. Main Chain.- C. Model Building.- D. The Side Chains.- E. Refinement of the Protein Structure.- VI. Correlation of the Chemical Sequence with the Electron Density Maps.- A. Structure Interpretation with Chemical Sequence.- B. Structure Interpretation with Partial Sequence.- VII. Conclusion.- 7 Amino Acid Sequence Determination by Mass Spectrometry.- I. Introduction.- II. Instrumentation and Techniques.- A. Ionization.- B. Analysis.- C. Resolution.- D. Sample Introduction.- III. Mass Spectrometry of Peptides.- A. Derivatization of Peptides.- B. Determination of Amino Acid Sequence from the Mass Spectrum.- C. Analysis of Peptide Mixtures.- IV. Concluding Remarks.- A. Recent Developments in Instrumentation.- B. Relationship of Mass Spectrometry to Conventional Methods for Sequence Analysis.- 8 Peptide Sequence Analysis by Nuclear Magnetic Resonance Spectroscopy.- I. Introduction.- II. NMR Principles.- III. Amino Acid Composition and Residue Identification.- A. Analysis of High Resolution 1H Spectra.- B. Analysis of High Resolution 13C Spectra.- C. Use of Paramagnetic Shift and Relaxation Enhancement (Broadening) Probes.- D. Sample Preparation.- IV. Conclusions.- References. 208 pp. Englisch.

Von Händler/Antiquariat, AHA-BUCH GmbH [51283250], Einbeck, Germany.
This item is printed on demand - Print on Demand Titel. Neuware - 1 Step-wise Degradation of Peptides Attached to Solid Supports.- I. Introduction.- II. Choice of Solid Support.- A. Polystyrene Beads.- B. Glass Beads.- III. Attachment of Peptides to Supports.- A. Carboxyl Activation Methods.- B. Amino Group Coupling.- IV. Step-wise Degradation Procedures.- A. Isothiocyanate Methods.- B. Thioacetylation Method.- V. Automation of Solid Phase Degradation.- A. Semi-Automated Operation.- B. An Inexpensive Automated Apparatus.- VI. Concluding Remarks.- 2 Coupling Methods and Strategies in Solid-Phase Sequencing.- I. Introduction.- II. Coupling Procedures.- A. Attachment by Activation of Carboxyl Groups.- B. Attachment of Amino Side Chains Using p-Phenylene Diisothiocyanate.- C. Combined Methods of Attachment.- D. Sequencing Resins.- E. Summary of Attachment Procedures.- III. Sequencing Strategies.- 3 Sequencing Peptides and Proteins Lacking Free -Amino Groups.- I. Introduction.- II. Isolation and Detection of Peptides Lacking Free -Amino Groups.- III. PCA-Terminating Peptides and Proteins.- A. Chemical Approaches.- B. Enzymatic Removal of PCA Residues.- IV. Acylated Peptides and Proteins.- A. Chemical Identification Procedures.- B. Enzymatic Removal of Acetylated Amino-Terminal Residues.- V. Concluding Remarks.- 4 Use of Antibody in the Study of Protein Structure.- I. Introduction.- II. Measurement of the Ag-Ab Reaction.- III. The Effect of Conformational Changes on the Antigenicity of Proteins.- IV. Detection of Sequence Changes in Proteins with Antibodies.- V. Use of Antibody in the Study of Protein Evolution.- 5 Polarization of Light and Protein Structure.- I. Introduction.- II. Plane Polarized Light.- III. Circularly Polarized Light.- IV. Circular Dichroism.- V. Drude Equation.- VI. Optical Properties of Amino Acids.- VII. Optical Properties of Peptides.- VIII. Conclusion.- 6 The X-ray Crystallography Technique in Protein Sequencing.- I. Introduction.- II. Outline of the Protein Crystallographic Method.- III. Some Important Concepts in X-ray Diffraction Analysis of Protein Crystals.- IV. Building up the Electron Density Map.- V. Interpretation of the Electron Density Map.- A. Molecular Boundary.- B. Main Chain.- C. Model Building.- D. The Side Chains.- E. Refinement of the Protein Structure.- VI. Correlation of the Chemical Sequence with the Electron Density Maps.- A. Structure Interpretation with Chemical Sequence.- B. Structure Interpretation with Partial Sequence.- VII. Conclusion.- 7 Amino Acid Sequence Determination by Mass Spectrometry.- I. Introduction.- II. Instrumentation and Techniques.- A. Ionization.- B. Analysis.- C. Resolution.- D. Sample Introduction.- III. Mass Spectrometry of Peptides.- A. Derivatization of Peptides.- B. Determination of Amino Acid Sequence from the Mass Spectrum.- C. Analysis of Peptide Mixtures.- IV. Concluding Remarks.- A. Recent Developments in Instrumentation.- B. Relationship of Mass Spectrometry to Conventional Methods for Sequence Analysis.- 8 Peptide Sequence Analysis by Nuclear Magnetic Resonance Spectroscopy.- I. Introduction.- II. NMR Principles.- III. Amino Acid Composition and Residue Identification.- A. Analysis of High Resolution 1H Spectra.- B. Analysis of High Resolution 13C Spectra.- C. Use of Paramagnetic Shift and Relaxation Enhancement (Broadening) Probes.- D. Sample Preparation.- IV. Conclusions.- References. 208 pp. Englisch.

Von Händler/Antiquariat, Phatpocket Limited [8420744], Waltham Abbey, HERTS, United Kingdom.
Ex-library, so some stamps and wear, but in good overall condition.

Von Händler/Antiquariat, ExtremelyReliable [8304062], RICHMOND, TX, U.S.A.
This item is printed on demand.

Von Händler/Antiquariat, Revaluation Books [2134736], Exeter, United Kingdom.
reprint edition. 210 pages. In Stock.