Studies in the Standardization of the Wassermann Reaction
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Von Händler/Antiquariat, profnath.
This historic book may have numerous typos and missing text. Purchasers can usually download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1919 edition. Excerpt: ...nonsyphilitic person may contain such slight amounts of these anticomplementary substances as to yield a satisfactory serum control of complete hemolysis and yet in the presence of an antigen show incomplete hemolysis, due to the summation of the so-called anticomplementary effects of serum and antigen, which separately were without demonstrable anticomplementary activity in the controls of the test. PURPOSES OF INVESTIGATION Ordinarily complement is titrated by mixing the complement serum, hemolysin and corpuscles at once and incubating at 38 C. for 'Investigation aided by funds accruing from the preparation of arsphcnamine. one hour; as shown in a previous article (2) some destruction of complement inevitably occurs during a primary incubation which is particularly marked when the complement is titrated in the presence of antigen with a primary incubation of four to eighteen hours at 8 C. to 10 C. with or without an additional incubation at 38 C. For this reason it is important to study the influence of temperature and duration of primary incubation upon nonspecific complement fixation or anticomplementary activity by antigen alone and serum alone, which constitute the purposes of this investigation in relation to studies bearing upon the primary incubation for complement-fixation tests. Part 1 THE INFLUENCE OF TEMPERATURE AND DURATION OF PRIMARY INCUBATION UPON THE ANTICOMPLEMENTARY ACTIVITY OF ANTIGENS Technic.--In conducting these experiments guinea pig complement was titrated plain and without primary incubation by mixing varying amounts of serum with constant amounts of antisheep hemolysin and corpuscles and water-bath incubation of one hour when the unit was read. Each organ extract was diluted 1:10 with physiologic saline... Paperback, Label: RareBooksClub.com, RareBooksClub.com, Produktgruppe: Book, Publiziert: 2013-09-13, Studio: RareBooksClub.com.

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Von Händler/Antiquariat, Amazon.com.
This historic book may have numerous typos and missing text. Purchasers can usually download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1919 edition. Excerpt: ...nonsyphilitic person may contain such slight amounts of these anticomplementary substances as to yield a satisfactory serum control of complete hemolysis and yet in the presence of an antigen show incomplete hemolysis, due to the summation of the so-called anticomplementary effects of serum and antigen, which separately were without demonstrable anticomplementary activity in the controls of the test. PURPOSES OF INVESTIGATION Ordinarily complement is titrated by mixing the complement serum, hemolysin and corpuscles at once and incubating at 38 C. for 'Investigation aided by funds accruing from the preparation of arsphcnamine. one hour; as shown in a previous article (2) some destruction of complement inevitably occurs during a primary incubation which is particularly marked when the complement is titrated in the presence of antigen with a primary incubation of four to eighteen hours at 8 C. to 10 C. with or without an additional incubation at 38 C. For this reason it is important to study the influence of temperature and duration of primary incubation upon nonspecific complement fixation or anticomplementary activity by antigen alone and serum alone, which constitute the purposes of this investigation in relation to studies bearing upon the primary incubation for complement-fixation tests. Part 1 THE INFLUENCE OF TEMPERATURE AND DURATION OF PRIMARY INCUBATION UPON THE ANTICOMPLEMENTARY ACTIVITY OF ANTIGENS Technic.--In conducting these experiments guinea pig complement was titrated plain and without primary incubation by mixing varying amounts of serum with constant amounts of antisheep hemolysin and corpuscles and water-bath incubation of one hour when the unit was read. Each organ extract was diluted 1:10 with physiologic saline... Paperback, Label: RareBooksClub.com, RareBooksClub.com, Produktgruppe: Book, Publiziert: 2013-09-13, Studio: RareBooksClub.com.

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John Albert Kolmer, Paperback, English-language edition, Pub by General Books LLC.